Mô tả

BEST ONLINE HPLC TRAINING IN THE WORLD!

High Performance Liquid Chromatography (HPLC) is a widely used analytical technique in the field of chemistry and biology. It is used to separate, identify and quantify components in a mixture. With the increasing demand for high-quality analysis, it is important to have the necessary skills and knowledge to operate HPLC equipment and interpret results accurately. This is where our HPLC Training comes in.

Our comprehensive training program is designed to provide you with a complete understanding of HPLC and its applications. Whether you are a beginner or an experienced analyst, our courses offer a comprehensive understanding of the principles and practices of HPLC. You will learn the logic of HPLC, such as how the device works, what the different parts of the equipment are and how to operate it safely.

One of the key features of our HPLC Training is that it is taught in a laboratory setting. This means you will see hands-on work with the equipment and learn how to apply a variety of analytical techniques. Also, our program includes 21 different analysis applications, so you can familiarize yourself with the various applications of HPLC and learn how to apply them in different scenarios.

Throughout the training, you will be taught by expert trainers who have years of experience in HPLC analysis. They will guide you through every step of the process, from the preparation of solutions and analytes to the analysis itself. You will learn how to analyze with different detectors, understand the different types of problems that may arise and how to solve them.

Our HPLC Training program also covers the software part of the device, so you will learn how to navigate and use the software to analyze data effectively. This includes mastering the calibration process, calibration curve creation, and the reporting and printing process.

By the end of the training, you will have a thorough understanding of HPLC and be able to analyze independently with HPLC devices. You will also have the necessary skills to manage a laboratory and be a lab manager. Whether you are looking to advance your career in the field of chemistry or biology, or simply want to gain a deeper understanding of HPLC, our training program is the perfect solution. Enroll now and take the first step towards becoming an HPLC expert.

Bạn sẽ học được gì

Comprehensive HPLC Knowledge: Participants will gain in-depth knowledge of HPLC technology.

Expert Training: Our expert trainers will guide participants in equipment usage, solution and analyte preparation, and analysis with different detectors.

Methodology and Process Understanding: A solid understanding of the methodologies and processes used in HPLC analysis.

Software Proficiency: Participants will develop proficiency in using the software associated with HPLC devices.

Problem-Solving Skills: Training will equip participants with the tools to address various challenges that may arise during analysis.

Independent Analysis: By the program's conclusion, participants will have the knowledge and confidence to conduct HPLC analysis independently.

Nuanced Understanding: Participants will grasp the intricacies of HPLC analysis, enabling them to analyze with confidence.

Yêu cầu

  • Basic Knowledge of Chemistry, About Analysis, and Math

Nội dung khoá học

24 sections

Mobile Phase And Washing Solutions

5 lectures
What is the Mobile Phase?
07:07
Wash Solvents and Lines
09:21
Recommendations For Different Washing Systems
02:49
How to Connect Mobile Phase to the System
08:32
Reverse Phase and Normal Phase
06:28

HPLC Pump Module

5 lectures
Operation System of Pump Module
02:50
How to Operate the Pump System?
04:14
How Should We Remove Air Bubbles Formed in the System?
07:33
The Liquid Sensor
04:13
The Pump Module in Different Brands of Devices
02:18

Auto Sampler Module

3 lectures
Auto Sampler Module
13:51
Sample Vials
06:20
How Does Work the Injection System
02:16

Column Oven Module

5 lectures
Column Oven
07:22
The Column Advice For Wash Process
07:50
HPLC Columns
08:21
How Assembled The Column?
02:22
What is the Guard Column?
03:10

Preliminary Information About HPLC Detectors and Software System

2 lectures
Detectors Used In HPLC Devices
09:16
Let’s Review The Software Program
08:14

Things to Do Before Analysis

11 lectures
Recommendations – Before Starting the Analysis
04:23
How Much Should the Mobile Phase Amount Be?
02:25
Preparation of the Mobile Phase
14:01
Recommendations
06:07
Things to Do Before Connecting the Mobile Phase and Washing Solutions
04:45
Characteristics of Columns
05:46
Pressure Problems Related to Columns
01:47
Operation and Washing Processes of The Device – Auto Purge 1
08:42
Cleaning the Pump Module
02:12
Starting Flow in the Pump Module
03:16
Connecting the Mobile Phase and Washing Solutions to the Device
03:03

Preparation of Samples and Analytes

7 lectures
Working Logic with Solutions
08:47
Preparation of Reference Solutions
04:33
Preparation of Stock Solution
05:05
Preparation of Sample Solution
04:18
Preparation of Stock Standard Solutions
05:25
Preparation of Vials
06:22
Placing Vials on the Tray
01:35

Method Creation and Method Definition In The Software

2 lectures
New method
21:33
Creating the Batch Table
13:47

How to Analyze with HPLC Device?

3 lectures
Conditioning the Device and Column
10:44
Starting to Analysis
07:17
Setting the Shutdown Washing Mode
02:21

Determination of Retention Times

4 lectures
Determination of Retention Times of Reference Standards (Automatic Integration)
10:21
Determination of Retention Times of Reference Standards (Manual Integration)
07:56
Determination of Vitamin C Standard
12:40
Determination of Vitamins B1, B3, B6
04:13

Calculation of Actual Concentrations

1 lectures
Calculation of Concentrations
03:31

Processing the Analysis

5 lectures
Introduction to Processing Analysis Results
05:54
Getting Started with the Process
11:28
Processing the Batch Table
13:46
Calling the Batch Table
02:27
Adding Parameters to the Result
01:17

Displaying the Calibration Curves

1 lectures
Displaying the Curves
06:49

Calculation Process

1 lectures
Calculation
09:27

Reporting, Calibration Curve And Print Process

5 lectures
Control of Concentrations Before Reporting
10:58
Report Preparation
11:18
Calibration Curve Deviate
05:47
Printing in a Different Format
04:07
Setting Peaks in a Report
04:14

Analysis in a Gradient System

1 lectures
Analysis in a Gradient System
10:57

Washing Processes / Auto Purge

2 lectures
Operation and Washing Processes of The Device Auto Purge Lesson 1
08:42
Washing Processes Auto Purge – Lesson 2
05:52

Mixing of Mobile Phases in Different Ways

2 lectures
Mixing in the Pump Line
20:02
Comparing Mixtures
06:46

Modifying the Method And Batch While the Analysis Is In Progress

2 lectures
Modifying the Method While the Analysis Is In Progress
02:17
Making Changes to the Batch Table While Analysis Is In Progress
01:02

Diode Array Detector || Analysis And Interpreting Results

3 lectures
Preparing Device and Lines Before Analysis in Diode Array Detector
11:14
Analysis in a Diode Array Detector
16:05
Interpreting Results in a Diode Array Detector
11:14

Analysis With RID Detector

1 lectures
Analysis in RID Detector
04:00

Different Columns With The Same Feature

1 lectures
Analysis in Fluorescent Detector
05:22

Different Columns With The Same Feature

2 lectures
Analysis with 25 cm Long Column
08:41
Long Column vs. Short Column
04:19

Problems That May Be Encountered And Solution Methods

9 lectures
Tailing Problem
03:00
Resolution – Dividing Peak Retention Times
01:17
If Parameters Change
02:01
If Flow Rate Changes
11:06
If the Wavelength Changes
04:36
If the UV Detector Lamp Stays Off
02:32
If Column Oven Temperature Changes
08:54
Lamp Life of UV Detector
03:05
If There Is a Difference in Mobile Phase Ratio
07:55

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